Faculty Collaboration Database

Pressure volume curves in arrested heterotopic rat heart isografts: role of improved myocardial protection. J Surg Res 1999 Sep;86(1):123-9

Date

08/24/1999

Pubmed ID

10452878

DOI

10.1006/jsre.1999.5683

Scopus ID

2-s2.0-0032821183 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

BACKGROUND: To minimize decreases in left ventricular (LV) compliance immediately after rat heart transplantation, we tested several different methods of myocardial protection.

MATERIALS AND METHODS: Five groups of ACI rat hearts (n = 6 each) were arrested by coronary perfusion with 5 ml of UW (University of Wisconsin), UW-BDM (UW with 2,3-butanedione monoxime), CU (Columbia University), or CU-BDM solution or by LV injection of potassium chloride and Ringer's lactate immersion (KCl/RL). After abdominal isografting and blood reperfusion for 15 min, transplanted hearts (TxH) were arrested and excised. Diastolic LV pressure-volume curves (LVPVCs) were correlated with myocardial water content (MWC). Native hearts (NH) were arrested identically to TxH and maintained at 4 degrees C by immersion. LVPVCs were measured at 15-min intervals for 90 min.

RESULTS: In three of four pressure intervals at Time 0, normalized LV volume (LVV) was smaller (P < 0.05, ANOVA) in KCl/RL native hearts than in the four perfusion groups. LVV decreased significantly in NH after 45-75 min; LVV decreased similarly with time in all groups. In TxH, postarrest LVVs were higher with UW-BDM, CU-BDM, and CU than with UW or KCl/RL (P < 0.05, ANOVA). Expressing LVV of TxH as a percentage of NH, UW-BDM, CU, and CU-BDM provided qualitatively better diastolic properties than KCl/RL and UW.

CONCLUSIONS: Thus rat LVPVCs can be improved after heart transplantation with alternative strategies of myocardial protection. KCl arrest decreases LV filling volume in this model and should be avoided.

Author List

Starr JP, Jia CX, Rabkin DG, Amirhamzeh MM, Hart JP, Hsu DT, Soto P, Pinsky D, Spotnitz HM

Author

Joseph Hart MD Associate Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Abdomen
Adenosine
Allopurinol
Animals
Blood Pressure
Blood Volume
Diacetyl
Glutathione
Heart Arrest, Induced
Heart Transplantation
Insulin
Isotonic Solutions
Myocardial Reperfusion
Organ Preservation Solutions
Potassium Chloride
Raffinose
Rats
Rats, Inbred ACI
Transplantation, Heterotopic
Ventricular Function, Left