Medical College of Wisconsin
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Advantages of preelectrophoretic conjugation of polypeptides with fluorescent dyes. Anal Biochem 1983 Jul 15;132(2):334-7

Date

07/15/1983

Pubmed ID

6414333

DOI

10.1016/0003-2697(83)90016-7

Scopus ID

2-s2.0-0021104426 (requires institutional sign-in at Scopus site)   35 Citations

Abstract

A rapid simple procedure is described for the conjugation of proteins, glycoproteins, and peptides with the fluorescent dye fluorescein isothiocyanate during the time required to polymerize a polyacrylamide gel. Such conjugation does not perturb the electrophoretic mobility of the polypeptides in detergent containing gels. The location of polypeptide . dye conjugate is evident by inspection immediately upon removal of a gel from an electrophoresis cabinet avoiding the time required for postelectrophoretic staining and destaining procedures. The sensitivity of detection of polypeptide . fluorescein conjugates is at least equivalent to that obtained using Coomassie blue.

Author List

Strottmann JM, Robinson JB Jr, Stellwagen E

Author

James M. Strottmann MD Associate Professor in the Radiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Electrophoresis, Polyacrylamide Gel
Fluorescein-5-isothiocyanate
Fluoresceins
Fluorescent Dyes
Glycoproteins
Peptides
Rosaniline Dyes
Thiocyanates