The transcriptional network controlling pluripotency in ES cells. Cold Spring Harb Symp Quant Biol 2008;73:195-202
Date
01/01/2008Pubmed ID
19478325DOI
10.1101/sqb.2008.72.001Scopus ID
2-s2.0-68549104469 (requires institutional sign-in at Scopus site) 75 CitationsAbstract
Embryonic stem (ES) cells are capable of continuous self-renewal and pluripotential differentiation. A "core" set of transcription factors, Oct4, Sox2, and Nanog, maintains the ES cell state, whereas various combinations of factors, invariably including Oct4 and Sox2, reprogram somatic cells to pluripotency. We have sought to define the transcriptional network controlling pluripotency in mouse ES cells through combined proteomic and genomic approaches. We constructed a protein interaction network surrounding Nanog and determined gene targets of the core and reprogramming factors, plus others. The expanded transcriptional network we have constructed forms the basis for further studies of directed differentiation and lineage reprogramming, and a paradigm for comprehensive elucidation of regulatory pathways in other stem cells.
Author List
Orkin SH, Wang J, Kim J, Chu J, Rao S, Theunissen TW, Shen X, Levasseur DNAuthor
Sridhar Rao MD, PhD Associate Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Line
Embryonic Stem Cells
Gene Regulatory Networks
Homeodomain Proteins
Mice
Models, Biological
Nanog Homeobox Protein
Octamer Transcription Factor-3
Pluripotent Stem Cells
Promoter Regions, Genetic
Proteomics
Transcription Factors
Transcription, Genetic
