Anion transport regulates intracellular pH in renal cortical tissue. Biochim Biophys Acta 1981 Oct 20;648(1):87-92
Date
10/20/1981Pubmed ID
6794625DOI
10.1016/0005-2736(81)90127-9Scopus ID
2-s2.0-0019770951 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
The regulation of cell pH by anion transport was examined in suspensions of rabbit renal proximal tubules. Values for cell pH were derived from 14C-labeled 5,5-dimethyloxazolidine-2,4-dione distribution. In buffer with 10 mM/l HCO3-- and gassed with 95% O2/5% CO2, the anion transport inhibitors, 4-acetamido-4'-isothiocyano-2,2'-disulfonic stilbene and furosemide, raised the cell-to-extracellular pH gradient from 0.23 +/- 0.02 to 0.31 +/- 0.02 and 0.31 +/- 0.03, respectively, but in combination their effects were not additive. Replacement of extracellular Cl-- by NO3-- raised the pH gradient from 0.24 +/- 0.04 to 0.37 +/- 0.05. Neither inhibitor raised the pH gradient in Cl-- -free media. Incubation of suspensions in HCO3-- and CO2-free media raised the pH gradient from 0.18 +/- 0.02 to 0.29 +/- 0.03. Removal of Cl-- in addition to HCO3-- and CO2 raised the pH gradient still further, to 0.36 +/- 0.02. The results demonstrate that two different anion transport inhibitors raise cell pH and the cell-to-extracellular pH gradient in proximal tubules and are consistent with the idea that the mechanism for this effect is inhibition of alkali anion exit from the tubule cell. This process appears to depend on extracellular Cl-- and probably occurs primarily by HCO3-- transport. The results support the concept that alkali anion transport, most probably HCO3-- exit from the peritubular cell border, is an important regulator of cell pH in renal proximal tubule.
Author List
Kleinman JG, Ware RA, Schwartz JHAuthor
Jack Kleinman MD Emeritus Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAnimals
Anions
Bicarbonates
Biological Transport
Carbon Dioxide
Chlorides
Furosemide
Hydrogen-Ion Concentration
Kidney Tubules
Rabbits
