Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells. Methods Mol Biol 2020;2097:107-113
Date
11/30/2019Pubmed ID
31776922DOI
10.1007/978-1-0716-0203-4_7Scopus ID
2-s2.0-85075740556 (requires institutional sign-in at Scopus site)Abstract
Recent advances in cancer immunotherapy emphasize the need for an efficient method to genetically modify effector lymphocytes to express exogenous "synthetic" genes. NK cells represent 10-20% of total lymphocytes in the peripheral blood of humans and play an essential role in clearing infections and malignant cells. A significant number of NK cells express and utilize non-clonotypic receptors that recognize cognate ligands expressed on a broad spectrum of target cells. Thus, NK cells can be utilized as potent immunotherapeutic tools with fewer limitations. Considerable amount of progress in improving effector functions through genetic manipulations has been centered around T cells. However, a similar technological and translational exploration on NK cells is lacking. One major constrain is the significantly low efficiency of lentiviral-mediated gene transductions into primary human or mouse NK cells. We found that dextran, a branched glucan polysaccharide, significantly improves the transduction efficiency of human and mouse primary NK cells. This highly reproducible methodology offers an approach that can help to improve gene delivery into NK cells and thereby cancer immunotherapy.
Author List
Nanbakhsh A, Malarkannan SAuthor
Subramaniam Malarkannan PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Proliferation
Cell Separation
Cells, Cultured
Dextrans
Humans
Lentivirus
Mice, Inbred C57BL
Transduction, Genetic