The Adult Murine Intestine is Dependent on Constitutive Laminin-γ1 Synthesis. Sci Rep 2019 Dec 17;9(1):19303
Date
12/19/2019Pubmed ID
31848396Pubmed Central ID
PMC6917708DOI
10.1038/s41598-019-55844-xScopus ID
2-s2.0-85076615345 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
Laminin-γ1 is required for early embryonic development; however, the need for laminin-γ1 synthesis in adulthood is unknown. A global and inducible mouse model of laminin-γ1 deficiency was generated to address this question. Genetic ablation of the Lamc1 gene in adult mice was rapidly lethal. Despite global Lamc1 gene deletion in tamoxifen-induced mutant mice, there was minimal change in total cardiac, pulmonary, hepatic or renal laminin protein. In contrast, laminin-γ1 was significantly depleted in the small intestines, which showed crypt hyperplasia and dissociation of villous epithelium from adjacent mesenchyme. We conclude that the physiologic requirement for laminin-γ1 synthesis in adult mice is dependent on a tissue-specific basal rate of laminin-γ1 turnover that results in rapid depletion of laminin-γ1 in the intestine.
Author List
Fields B, DeLaForest A, Zogg M, May J, Hagen C, Komnick K, Wieser J, Lundberg A, Weiler H, Battle MA, Carlson KSAuthors
Karen-Sue B. Carlson MD, PhD Associate Professor in the Medicine department at Medical College of WisconsinHartmut Weiler PhD Associate Professor in the Physiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBasement Membrane
Embryonic Development
Female
Intestines
Laminin
Liver
Mice