Faculty Collaboration Database

Neutralizing epitopes of human parainfluenza virus type 3 are conformational and cannot be imitated by synthetic peptides. Vaccine 1991 Apr;9(4):243-9

Date

04/01/1991

Pubmed ID

1711742

DOI

10.1016/0264-410x(91)90107-h

Scopus ID

2-s2.0-0026016365 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

The possibility that linear epitopes on the haemagglutinin-neuraminidase (HN) surface glycoprotein of human parainfluenza virus type 3 (PIV-3) might induce neutralizing antibodies after virus infection was investigated. Thirty-seven peptides, representing 64% of the extramembranous portion of the HN molecule of PIV-3, were synthesized. Their ability to bind to 14 neutralizing murine monoclonal antibodies (mAbs) specific for HN or 26 high-titre human serum samples were tested in a direct enzyme-linked immunosorbent assay (ELISA) and in an indirect competition ELISA. None of the synthetic peptides reacted with any of the mAbs or serum samples in the direct test and none of 11 synthetic peptides tested blocked mAbs from binding to HN in the competition ELISA. These findings suggest that synthetic peptides cannot be used to imitate the known neutralizing epitopes on the HN. Analyses of reduced and non-reduced HN in ELISA and immunoblot assays confirmed that protein folding and tertiary structure are essential for epitope formation in these neutralizing sites. However, some children's sera analysed by immunoblotting contained antibodies to an uncharacterized linear epitope(s) not recognized by our panel of mAbs, raising the possibility that a neutralizing linear epitope does exist on the HN of PIV-3.

Author List

Henrickson KJ, Kingsbury DW, van Wyke Coelingh KL, Naeve CW, Portner A

Author

Kelly J. Henrickson MD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Animals
Antigens, Viral
Blotting, Western
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Epitopes
HN Protein
Haplorhini
Humans
Molecular Sequence Data
Parainfluenza Virus 3, Human
Peptides
Protein Conformation
Vaccines, Synthetic