Sodium gradient-driven transport processes in ATP-depleted renal tubules. Am J Physiol 1983 May;244(5):C331-5
Date
05/01/1983Pubmed ID
6846524DOI
10.1152/ajpcell.1983.244.5.C331Scopus ID
2-s2.0-0020758187 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
The effects of directed Na+ gradients on proximal tubule cell transport processes were examined in suspensions of rabbit renal tubules depleted of ATP. Cells of high-Na+ content were generated by suspending the tubules in high-Na+ media, whereas low-Na+ cells were produced by incubating tubules in Na+-free media. Resuspension of the high-Na+ tubules in Na+-free media caused a fall in cell pH simultaneous with a fall in cell Na+. Resuspending the low-Na+ cells in Na+-replete media led to a rise in cell pH, in parallel with the rise in cell Na+. Removing HCO-3 and CO2 augmented and amiloride inhibited the increase in cell pH generated by the inward Na+ gradient. Low-Na+ tubules exposed to an inwardly directed Na+ gradient also concentrated the sugar analogue alpha-methylglucoside, and this uptake was blocked by phlorizin. These findings demonstrate the suitability of ATP-depleted renal tubules for the study of linked transport processes by providing evidence for the existence of the proximal luminal transport processes, Na+-H+ exchange, and Na+-sugar cotransport in this preparation.
Author List
Blumenthal SS, Ware RA, Kleinman JGAuthors
Samuel S. Blumenthal MD Professor in the Medicine department at Medical College of WisconsinJack Kleinman MD Emeritus Professor in the Medicine department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Adenosine TriphosphateAmiloride
Animals
Biological Transport, Active
Buffers
Hydrogen-Ion Concentration
Kidney Cortex
Kidney Tubules
Methylglucosides
Rabbits
Sodium
