Faculty Collaboration Database

Glucoamylase activity in infants and children: normal values and relationship to symptoms and histological findings. J Pediatr Gastroenterol Nutr 2004 Aug;39(2):161-5

Date

07/23/2004

Pubmed ID

15269621

DOI

10.1097/00005176-200408000-00007

Scopus ID

2-s2.0-16644373937 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

BACKGROUND: Starch digestion is dependent on a combination of pancreatic and salivary amylase and the intestinal brush border enzymes glucoamylase and sucrase-isomaltase. Glucoamylase splits successive glucose molecules from the nonreducing end of starch molecules and is particularly important in infants who are relatively deficient in pancreatic amylase.

METHODS: The authors measured glucoamylase activity in endoscopic mucosal biopsies submitted for measurement of disaccharidase activity from 214 patients aged 1 month to 20 years. Glucoamylase activity was measured using glucose polymers (polycose) as the substrate. The authors also related enzyme activity to histologic appearance and clinical indication for endoscopy.

RESULTS: The most common reasons for biopsy were abdominal pain, gastroesophageal reflux, and vomiting. Disaccharidase activity by age was similar to previous reports. Glucoamylase activity did not differ with age, but was 2 to 3 times the activity reported previously. Glucoamylase activity was significantly depressed in children with the most severe histologic abnormalities. Normal glucoamylase activity (+/-2 SD) was 80.6 +/- 54.8 micromoles of glucose produced per minute per gram of protein.

CONCLUSIONS: Glucoamylase activity is 2 to 3 times higher when glucose polymers are used as substrate than when glycogen is used. Severe mucosal disease is associated with reduced glucoamylase activity. Quantitation of glucoamylase activity with glucose polymers is more appropriate in evaluating children since these polymers are commonly used as carbohydrate source in the diet.

Author List

Lee PC, Werlin S, Trost B, Struve M

Author

Steven L. Werlin MD Emeritus Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Age Factors
Child
Child, Preschool
Endoscopy, Gastrointestinal
Female
Glucan 1,4-alpha-Glucosidase
Glycogen
Humans
Infant
Infant, Newborn
Intestinal Absorption
Intestinal Mucosa
Intestine, Small
Lactase
Male
Polymers
Reference Values
Starch