Synthetic peptide approach to the analysis of kinase activities of avian EGF receptor and v-erbB protein. Biochimie 1985;67(10-11):1095-101
Date
10/01/1985Pubmed ID
3000459DOI
10.1016/s0300-9084(85)80106-1Scopus ID
2-s2.0-0022395830 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Analysis of the structure and function of a protein such as the epidermal growth factor receptor is facilitated by the use of antibodies directed against discrete portions of the protein. Here, we describe the characterization and use of antibodies directed against synthetic peptides corresponding to specific portions of the epidermal growth factor receptor and/or v-erbB protein. In particular, one useful antiserum has allowed us to compare the protein kinase activities of the epidermal growth factor receptor and the v-erbB proteins and to conclude that the v-erbB protein is a protein-tyrosine specific kinase as is its homologue the avian epidermal growth factor receptor.
Author List
Kris RM, Lax I, Sasson I, Copf B, Werlin S, Gullick W, Waterfield MD, Ullrich A, Fridkin M, Schlessinger JAuthor
Steven L. Werlin MD Emeritus Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AlpharetrovirusAnimals
Cell Membrane
Chickens
Cytoplasm
ErbB Receptors
Immune Sera
Immunosorbent Techniques
Molecular Weight
Peptide Fragments
Phosphorylation
Protein Kinases
Protein-Tyrosine Kinases
Radioimmunoassay
Receptors, Cell Surface
Viral Proteins
