Anaerobic bacterial populations on normal and diseased human biopsy tissue obtained at colonoscopy. Appl Environ Microbiol 1982 May;43(5):1173-81
Date
05/01/1982Pubmed ID
7103479Pubmed Central ID
PMC244202DOI
10.1128/aem.43.5.1173-1181.1982Scopus ID
2-s2.0-0019973186 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
Human epithelium was cultured to characterize differences in microbial populations between regions of normal colon and between polyps, inflammatory bowel disease, and cancer and their respective adjacent normal mucosa. Twenty-one patients (12 polyps, 5 inflammatory bowel disease, 4 cancer) underwent colonoscopy with anaerobic culture of mucosal biopsies from normal and diseased ascending, transverse, descending, and sigmoid colon. No differences for total number of organisms and recovery of species between ascending colon and other normal regions were seen except for sigmoid colon. Significant differences between polyps and adjacent normal tissue were seen for total number of organisms and recovery of genera and species. No significant differences in total number of organisms and recovery of genera were seen between cancer and inflammatory bowel disease and their respective adjacent normal tissue. The recovery of genera from polyps and normal tissue was Bacteroides greater than Fusobacterium greater than Clostridium greater than Eubacterium greater than Peptostreptococcus. These data suggest that (i) the total number of anaerobic organisms and species remained relatively constant, except for lower numbers in normal distal colon which were probably a result of the preparation for colonoscopy; (ii) polyp formation favored increased microbial colonization; and (iii) the increased number of organisms generally reflected those genera and species seen on adjacent normal mucosa.
Author List
Edmiston CE Jr, Avant GR, Wilson FAAuthor
Charles Edmiston MD, PhD Emeritus Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnaerobiosisBacteria
Bacteroidaceae
Clostridium
Colon
Colonic Neoplasms
Colonoscopy
Humans
Inflammation
Intestinal Diseases
Intestinal Mucosa
Peptococcaceae
Polyps
