Cytotoxicity of NiO and Ni(OH)2 Nanoparticles Is Mediated by Oxidative Stress-Induced Cell Death and Suppression of Cell Proliferation. Int J Mol Sci 2020 Mar 28;21(7)
Date
04/02/2020Pubmed ID
32231169Pubmed Central ID
PMC7178005DOI
10.3390/ijms21072355Scopus ID
2-s2.0-85082730687 (requires institutional sign-in at Scopus site) 19 CitationsAbstract
The use of nanomaterial-based products continues to grow with advancing technology. Understanding the potential toxicity of nanoparticles (NPs) is important to ensure that products containing them do not impose harmful effects to human or environmental health. In this study, we evaluated the comparative cytotoxicity between nickel oxide (NiO) and nickel hydroxide (Ni(OH)2) in human bronchoalveolar carcinoma (A549) and human hepatocellular carcinoma (HepG2) cell lines. Cellular viability studies revealed cell line-specific cytotoxicity in which nickel NPs were toxic to A549 cells but relatively nontoxic to HepG2 cells. Time-, concentration-, and particle-specific cytotoxicity was observed in A549 cells. NP-induced oxidative stress triggered dissipation of mitochondrial membrane potential and induction of caspase-3 enzyme activity. The subsequent apoptotic events led to reduction in cell number. In addition to cell death, suppression of cell proliferation played an essential role in regulating cell number. Collectively, the observed cell viability is a function of cell death and suppression of proliferation. Physical and chemical properties of NPs such as total surface area and metal dissolution are in agreement with the observed differential cytotoxicity. Understanding the properties of NPs is essential in informing the design of safer materials.
Author List
Cambre MH, Holl NJ, Wang B, Harper L, Lee HJ, Chusuei CC, Hou FYS, Williams ET, Argo JD, Pandey RR, Huang YWAuthor
Fang Yao Stephen Hou PhD, MB(ASCP)QCYM, MLS(ASCPi) Clinical Assistant Professor in the Biomedical Sciences Laboratory Programs department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
A549 CellsCell Death
Cell Proliferation
Cell Survival
Hep G2 Cells
Humans
Hydroxides
Nanoparticles
Nickel
Oxidative Stress
