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A platform for high-throughput bioenergy production phenotype characterization in single cells. Sci Rep 2017 03 28;7:45399

Date

03/30/2017

Pubmed ID

28349963

Pubmed Central ID

PMC5368665

DOI

10.1038/srep45399

Scopus ID

2-s2.0-85016622569   7 Citations

Abstract

Driven by an increasing number of studies demonstrating its relevance to a broad variety of disease states, the bioenergy production phenotype has been widely characterized at the bulk sample level. Its cell-to-cell variability, a key player associated with cancer cell survival and recurrence, however, remains poorly understood due to ensemble averaging of the current approaches. We present a technology platform for performing oxygen consumption and extracellular acidification measurements of several hundreds to 1,000 individual cells per assay, while offering simultaneous analysis of cellular communication effects on the energy production phenotype. The platform comprises two major components: a tandem optical sensor for combined oxygen and pH detection, and a microwell device for isolation and analysis of single and few cells in hermetically sealed sub-nanoliter chambers. Our approach revealed subpopulations of cells with aberrant energy production profiles and enables determination of cellular response variability to electron transfer chain inhibitors and ion uncouplers.

Author List

Kelbauskas L, Glenn H, Anderson C, Messner J, Lee KB, Song G, Houkal J, Su F, Zhang L, Tian Y, Wang H, Bussey K, Johnson RH, Meldrum DR

Author

Roger H. Johnson PhD Associate Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Bioreactors
Cell Communication
Cell Line, Tumor
Cell Survival
Energy Metabolism
Humans
Oxidative Phosphorylation
Oxygen
Oxygen Consumption