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A new highly selective fluorescent K+ sensor. J Am Chem Soc 2011 Nov 23;133(46):18530-3

Date

10/27/2011

Pubmed ID

22026580

Pubmed Central ID

PMC3223084

DOI

10.1021/ja207345s

Scopus ID

2-s2.0-83055161605   118 Citations

Abstract

We describe the synthesis, properties, and application of a new fluorescent potassium chemosensor, KS2, for K(+) sensing and imaging in live cells. By virtue of a strong electron-withdrawing group, 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF), with a triazacryptand ligand, the new sensor can respond to K(+) up to 1.6 M. This is the first highly selective intracellular sensor suitable for sensing K(+) over a broad and high concentration range. Confocal fluorescence microscopy has established the utility of KS2 for live-cell K(+) detection. The application of KS2 combined with other sensors will be of great benefit for investigating cellular metabolism, detecting and diagnosing diseases including cancer, and monitoring responses to therapy.

Author List

Zhou X, Su F, Tian Y, Youngbull C, Johnson RH, Meldrum DR

Author

Roger H. Johnson PhD Associate Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cell Line, Tumor
Cells, Cultured
Fluorescent Dyes
Humans
Microscopy, Confocal
Models, Molecular
Molecular Structure
Potassium