Faculty Collaboration Database

Placental endothelin-converting enzyme-1 is decreased in preeclampsia. Pregnancy Hypertens 2020 Apr;20:108-110

Date

04/12/2020

Pubmed ID

32278308

Pubmed Central ID

PMC7532811

DOI

10.1016/j.preghy.2020.04.001

Scopus ID

2-s2.0-85082836517 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

Endothelin-converting enzyme-1(ECE-1) is a key regulatory enzyme in the processing of endothelin-1 (ET-1). We quantified and localized ECE-1 in normal and preeclamptic placentas. Normal (n=6) and preeclamptic (n=6) placentas were serially sectioned for immunofluorescence (IF). Cell type specific markers identified endothelial, trophoblast, macrophage, smooth muscle, and fibroblast cells. Quantitative analyses were performed by western blot and ELISA. IF identified ECE-1 expression within the stroma and villous space. Cellular localization of ECE-1 was limited to endothelial membranes. There was significantly less ECE-1 in preeclamptic placentas, suggesting ECE-1 is important for proper regulation of ET-1 within the placenta.

Author List

McCormick A, Kristianto J, Wang X, McIntosh J, Cruz M, Hibbard JU, Blank RD

Authors

Robert D. Blank MD, PhD Emeritus Professor in the Medicine department at Medical College of Wisconsin
Meredith Cruz MD Professor in the Obstetrics and Gynecology department at Medical College of Wisconsin
Jennifer Jury Mcintosh DO Associate Professor in the Obstetrics and Gynecology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Biomarkers
Case-Control Studies
Chorionic Villi
Down-Regulation
Endothelial Cells
Endothelin-Converting Enzymes
Female
Humans
Placenta
Pre-Eclampsia
Pregnancy
Stromal Cells