Comparison of immune responses and protective efficacy of suicidal DNA vaccine and conventional DNA vaccine encoding glycoprotein C of pseudorabies virus in mice. Vaccine 2004 Jan 02;22(3-4):345-51
Date
12/13/2003Pubmed ID
14670315DOI
10.1016/j.vaccine.2003.08.010Scopus ID
2-s2.0-0344665683 (requires institutional sign-in at Scopus site) 53 CitationsAbstract
In the present study, the immunogenicity and protective efficacy of a suicidal DNA vaccine (pSFVC1.5) incorporating Semliki Forest virus (SFV) replicon and expressing glycoprotein C (gC) of pseudorabies virus (PrV) was investigated and compared with a conventional plasmid DNA vaccine (pcDC) encoding the same antigen. In vitro, pSFVC1.5 could express gC protein and induce apoptosis of the transfected cells. After immunization in BALB/c mice, the gC-specific ELISA antibodies and neutralizing antibodies induced by pSFVC1.5 were relatively lower than those obtained in mice immunized with pcDC. However, mice immunized with pSFVC1.5 could confer more efficient protection than pcDC (100 and 62.5%, respectively) when challenged with the field PrV at 4 weeks after secondary immunization. Further analyses of cell-mediated immune responses showed that pSFVC1.5 induced stronger lymphocyte proliferative responses and higher levels of IFN-gamma, suggesting pSFVC1.5 could induce an enhanced Th1-type immune response. Collectively these results indicated that suicidal DNA vaccine is an alternative strategy to conventional DNA vaccine and can be considered a promising approach for the development of an efficacious vaccine against PrV.
Author List
Xiao S, Chen H, Fang L, Liu C, Zhang H, Jiang Y, Hong WAuthor
Wenzhou Hong DVM, PhD Assistant Professor in the Otolaryngology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies, Viral
Antibody Formation
Antigens, Viral
Apoptosis
Cell Division
DNA Fragmentation
Enzyme-Linked Immunosorbent Assay
Female
Herpesvirus 1, Suid
Immune Tolerance
Immunity, Cellular
Interferon-gamma
Lymphocytes
Mice
Mice, Inbred BALB C
Neutralization Tests
Plasmids
Pseudorabies
Pseudorabies Vaccines
Transfection
Vaccines, DNA
Viral Envelope Proteins