Satellite glial cells in sensory ganglia express functional transient receptor potential ankyrin 1 that is sensitized in neuropathic and inflammatory pain. Mol Pain 2020;16:1744806920925425
Date
06/03/2020Pubmed ID
32484015Pubmed Central ID
PMC7268132DOI
10.1177/1744806920925425Scopus ID
2-s2.0-85085909943 (requires institutional sign-in at Scopus site) 40 CitationsAbstract
Transient receptor potential ankyrin 1 (TRPA1) is well documented as an important molecule in pain hypersensitivity following inflammation and nerve injury and in many other cellular biological processes. Here, we show that TRPA1 is expressed not only by sensory neurons of the dorsal root ganglia (DRG) but also in their adjacent satellite glial cells (SGCs), as well as nonmyelinating Schwann cells. TRPA1 immunoreactivity is also detected in various cutaneous structures of sensory neuronal terminals, including small and large caliber cutaneous sensory fibers and endings. The SGC-expressed TRPA1 is functional. Like DRG neurons, dissociated SGCs exhibit a robust response to the TRPA1-selective agonist allyl isothiocyanate (AITC) by an increase of intracellular Ca2+ concentration ([Ca2+]i). These responses are abolished by the TRPA1 antagonist HC030031 and are absent in SGCs and neurons from global TRPA1 null mice. SGCs and neurons harvested from DRG proximal to painful tissue inflammation induced by plantar injection of complete Freund's adjuvant show greater AITC-evoked elevation of [Ca2+]i and slower recovery compared to sham controls. Similar TRPA1 sensitization occurs in both SGCs and neurons during neuropathic pain induced by spared nerve injury. Together, these results show that functional TRPA1 is expressed by sensory ganglia SGCs, and TRPA1 function in SGCs is enhanced after both peripheral inflammation and nerve injury, and suggest that TRPA1 in SGCs may contribute to inflammatory and neuropathic pain.
Author List
Shin SM, Itson-Zoske B, Cai Y, Qiu C, Pan B, Stucky CL, Hogan QH, Yu HAuthors
Quinn H. Hogan MD Professor in the Anesthesiology department at Medical College of WisconsinSeung Min Shin BS, PhD, MS Assistant Professor in the Anesthesiology department at Medical College of Wisconsin
Cheryl L. Stucky PhD Professor in the Cell Biology Neurobiology and Anatomy department at Medical College of Wisconsin
Hongwei Yu MD Professor in the Anesthesiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCell Size
Ganglia, Spinal
Inflammation
Isothiocyanates
Male
Mice, Inbred C57BL
Neuralgia
Neuroglia
Neurons
Rats, Sprague-Dawley
Schwann Cells
Sensory Receptor Cells
TRPA1 Cation Channel
