Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior. Leukemia 2006 Oct;20(10):1855-62
Date
08/11/2006Pubmed ID
16900213DOI
10.1038/sj.leu.2404345Scopus ID
2-s2.0-33750060054 (requires institutional sign-in at Scopus site) 68 CitationsAbstract
Non-Hodgkin's lymphoma (NHL) is a group of malignancies of the immune system with variable clinical behaviors and diverse molecular features. Despite the progress made in classification of NHLs based on classical methods, molecular classifications are a work in progress. Toward this goal, we used an array-based technique called differential methylation hybridization (DMH) to study small B-cell lymphoma (SBCL) subtypes. A total of 43 genomic DMH experiments were performed. From these results, several statistical methods were used to generate a set of differentially methylated genes for further validation. Methylation of LHX2, POU3F3, HOXC10, NRP2, PRKCE, RAMP, MLLT2, NKX6.1, LRP1B and ARF4 was validated in cell lines and patient samples and demonstrated subtype-related preferential methylation patterns. For LHX2 and LRP1B, bisulfite sequencing, real-time reverse transcriptase-polymerase chain reaction and induction of gene expression following treatment with the demethylating agent, 5'-aza-2'-deoxycytidine, were confirmed. This new epigenetic information is helping to define molecular portraits of distinct subtypes of SBCL that are not recognized by current classification systems and provides valuable potential insights into the biology of these tumors.
Author List
Rahmatpanah FB, Carstens S, Guo J, Sjahputera O, Taylor KH, Duff D, Shi H, Davis JW, Hooshmand SI, Chitma-Matsiga R, Caldwell CWAuthor
Sam I. Hooshmand DO Assistant Professor in the Neurology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultCell Line, Tumor
Cluster Analysis
CpG Islands
DNA Methylation
Epigenesis, Genetic
Female
Gene Expression Regulation, Neoplastic
Genomics
Homeodomain Proteins
Humans
LIM-Homeodomain Proteins
Leukemia, Lymphocytic, Chronic, B-Cell
Lymphoma, B-Cell
Male
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
RNA, Messenger
Receptors, LDL
Sulfites
Transcription Factors
