Polymer semiconductors as a biosensing platform: peroxidase activity of enzyme bound to organic semiconducting films. Annu Int Conf IEEE Eng Med Biol Soc 2007;2007:107-10
Date
11/16/2007Pubmed ID
18001900DOI
10.1109/IEMBS.2007.4352234Scopus ID
2-s2.0-57649143402 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Organic polymer semiconductors have unique electronic properties which make them attractive for use in microelectronic and optoelectronic devices fabricated using inexpensive manufacturing processes. In addition, novel chemical and biological sensors have been proposed which make use of the photophysical and electrical properties of conjugated polymer semiconducting films. The work described herein illustrates one such biosensing application by demonstrating successful immobilization of horseradish peroxidase enzyme onto a thin film of the semiconducting polymer MDMO-PPV. Validation of bound peroxidase activity is accomplished through the use of a substrate solution of 3,3'-diaminobenzidine and hydrogen peroxide, which yields a dark brown precipitate in the presence of peroxidase. Photometric measurements are used to derive a quantitative assay of bound peroxidase concentration. This work supports the feasibility of organic semiconducting polymer films as a biosensing platform in microelectronic sensor devices.
Author List
Omari EA, Klemer DP, Steeber DA, Gaertner WFAuthor
Eenas Omari PhD Associate Professor in the Radiation Oncology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
3,3'-DiaminobenzidineBiosensing Techniques
Colorimetry
Enzymes, Immobilized
Furans
Horseradish Peroxidase
Photometry
Polyvinyls
Semiconductors
