Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma. World J Gastroenterol 2011 Nov 28;17(44):4917-21
Date
12/16/2011Pubmed ID
22171134Pubmed Central ID
PMC3235636DOI
10.3748/wjg.v17.i44.4917Scopus ID
2-s2.0-83155177127 (requires institutional sign-in at Scopus site) 49 CitationsAbstract
AIM: To detect the effects of plasma DNA methylation of Wnt antagonists/inhibitors on recurrence of esophageal squamous cell carcinoma (ESCC).
METHODS: We used methylation-specific polymerase chain reaction to detect hypermethylation of the promoter of four Wnt antagonists/inhibitors (SFRP-1, WIF-1, DKK-3 and RUNX3) using DNA from the plasma of ESCC patients (n = 81) and analyzed the association between promoter hypermethylation of Wnt pathway modulator genes and the two-year recurrence of ESCC.
RESULTS: Hypermethylation of SFRP-1, DKK-3 and RUNX-3 was significantly associated with an increased risk of ESCC recurrence (P = 0.001, 0.003 and 0.001 for SFRP-1, DKK-3 and RUNX3, respectively). Patients carrying two to three methylated genes had a significantly elevated risk of recurrence compared with those not carrying methylated genes (odds ratio = 15.69, 95% confidential interval: 2.97-83). The area under the receiver operating characteristic curve (AUC) was 77.1 for ESCC recurrence prediction (sensitivity = 66.67 and specificity = 83.3). When combining methylated genes and the clinical stage, the AUC was 83.69, with a sensitivity of 76.19 and a specificity of 83.3.
CONCLUSION: The status of promoter hypermethylation of Wnt antagonists/inhibitors in plasma may serve as a non-invasive prognostic biomarker for ESCC.
Author List
Liu JB, Qiang FL, Dong J, Cai J, Zhou SH, Shi MX, Chen KP, Hu ZBAuthor
Jing Dong PhD Assistant Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adaptor Proteins, Signal TransducingAdult
Aged
Aged, 80 and over
Biomarkers, Tumor
Carcinoma, Squamous Cell
Chemokines
Core Binding Factor Alpha 3 Subunit
DNA Methylation
Esophageal Neoplasms
Female
Humans
Intercellular Signaling Peptides and Proteins
Male
Membrane Proteins
Middle Aged
Promoter Regions, Genetic
ROC Curve
Repressor Proteins
Secondary Prevention
Wnt Proteins
Wnt Signaling Pathway