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Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. PLoS One 2020;15(7):e0235852

Date

07/07/2020

Pubmed ID

32628717

Pubmed Central ID

PMC7337293

DOI

10.1371/journal.pone.0235852

Scopus ID

2-s2.0-85087672966

Abstract

The medulloblastoma (MB) microenvironment is diverse, and cell-cell interactions within this milieu is of prime importance. Astrocytes, a major component of the microenvironment, have been shown to impact primary tumor cell phenotypes and metastasis. Based on proximity of MB cells and astrocytes in the brain microenvironment, we investigated whether astrocytes may influence MB cell phenotypes directly. Astrocyte conditioned media (ACM) increased Daoy MB cell invasion, adhesion, and in vivo cellular protrusion formation. ACM conditioning of MB cells also increased CD133 surface expression, a key cancer stem cell marker of MB. Additional neural stem cell markers, Nestin and Oct-4A, were also increased by ACM conditioning, as well as neurosphere formation. By knocking down CD133 using short interfering RNA (siRNA), we showed that ACM upregulated CD133 expression in MB plays an important role in invasion, adhesion and neurosphere formation. Collectively, our data suggests that astrocytes influence MB cell phenotypes by regulating CD133 expression, a key protein with defined roles in MB tumorgenicity and survival.

Author List

Gronseth E, Gupta A, Koceja C, Kumar S, Kutty RG, Rarick K, Wang L, Ramchandran R

Authors

Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin
Kevin Richard Rarick PhD Assistant Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

AC133 Antigen
Animals
Astrocytes
Cell Adhesion
Cell Line, Tumor
Cell Movement
Cells, Cultured
Culture Media, Conditioned
Humans
Medulloblastoma
Neoplastic Stem Cells
Nestin
Octamer Transcription Factor-3
Phenotype
Tumor Microenvironment
Zebrafish
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d