Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. PLoS One 2020;15(7):e0235852
Date
07/07/2020Pubmed ID
32628717Pubmed Central ID
PMC7337293DOI
10.1371/journal.pone.0235852Scopus ID
2-s2.0-85087672966 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
The medulloblastoma (MB) microenvironment is diverse, and cell-cell interactions within this milieu is of prime importance. Astrocytes, a major component of the microenvironment, have been shown to impact primary tumor cell phenotypes and metastasis. Based on proximity of MB cells and astrocytes in the brain microenvironment, we investigated whether astrocytes may influence MB cell phenotypes directly. Astrocyte conditioned media (ACM) increased Daoy MB cell invasion, adhesion, and in vivo cellular protrusion formation. ACM conditioning of MB cells also increased CD133 surface expression, a key cancer stem cell marker of MB. Additional neural stem cell markers, Nestin and Oct-4A, were also increased by ACM conditioning, as well as neurosphere formation. By knocking down CD133 using short interfering RNA (siRNA), we showed that ACM upregulated CD133 expression in MB plays an important role in invasion, adhesion and neurosphere formation. Collectively, our data suggests that astrocytes influence MB cell phenotypes by regulating CD133 expression, a key protein with defined roles in MB tumorgenicity and survival.
Author List
Gronseth E, Gupta A, Koceja C, Kumar S, Kutty RG, Rarick K, Wang L, Ramchandran RAuthors
Suresh Kumar PhD Associate Professor in the Pathology department at Medical College of WisconsinRamani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin
Kevin Richard Rarick PhD Assistant Professor in the Pediatrics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AC133 AntigenAnimals
Astrocytes
Cell Adhesion
Cell Line, Tumor
Cell Movement
Cells, Cultured
Culture Media, Conditioned
Humans
Medulloblastoma
Neoplastic Stem Cells
Nestin
Octamer Transcription Factor-3
Phenotype
Tumor Microenvironment
Zebrafish