Faculty Collaboration Database

Expression of IGF-II and IGF binding proteins in differentiating human intestinal Caco-2 cells. Am J Physiol 1995 Nov;269(5 Pt 1):E804-13

Date

11/01/1995

Pubmed ID

7491929

DOI

10.1152/ajpendo.1995.269.5.E804

Scopus ID

2-s2.0-0028841779 (requires institutional sign-in at Scopus site)   29 Citations

Abstract

The mitogenic and metabolic effects of insulin-like growth factor-II (IGF-II) can be modulated by six distinct IGF binding proteins (IGFBPs). As a first step toward understanding the role of IGFs and their binding proteins in intestinal epithelial cell differentiation, the expression of IGF-II and IGFBPs was characterized in the human colon adenocarcinoma Caco-2 cell line. Northern blot analysis revealed two IGF-II transcripts of 5.4 and 4.5 kb, and ribonuclease protection assays indicated that IGF-II mRNA levels are regulated during Caco-2 differentiation. A specific radioimmunoassay detected IGF-II in serum-free conditioned medium, the level of which was three- to fivefold higher in proliferating cells than in differentiated cells. Immunoprecipitation and ligand blot analyses of conditioned medium demonstrated that IGFBP-2, IGFBP-3, IGFBP-4, and IGFBP-6 are synthesized by Caco-2 cells, with IGFBP-2 and IGFBP-4 being the major IGFBPs secreted, and that the levels of IGFBP-2 and IGFBP-6 decreased as differentiation proceeded. These results indicate that the expression of IGF-II, IGFBP-2, and IGFBP-6 is regulated in a differentiation-dependent manner in Caco-2 cells.

Author List

Zhang Y, Wick DA, Seetharam B, Dahms NM

Author

Nancy M. Dahms PhD Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Caco-2 Cells
Cell Differentiation
Cell Division
Gene Expression
Humans
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor II
Intestinal Mucosa
Intestines