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Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 2009 May 22;324(5930):1068-71

Date

05/02/2009

Pubmed ID

19407142

Pubmed Central ID

PMC2827199

DOI

10.1126/science.1173041

Scopus ID

2-s2.0-66249110335   1352 Citations

Abstract

Type 2C protein phosphatases (PP2Cs) are vitally involved in abscisic acid (ABA) signaling. Here, we show that a synthetic growth inhibitor called pyrabactin functions as a selective ABA agonist. Pyrabactin acts through PYRABACTIN RESISTANCE 1 (PYR1), the founding member of a family of START proteins called PYR/PYLs, which are necessary for both pyrabactin and ABA signaling in vivo. We show that ABA binds to PYR1, which in turn binds to and inhibits PP2Cs. We conclude that PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs. Our results illustrate the power of the chemical genetic approach for sidestepping genetic redundancy.

Author List

Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow TF, Alfred SE, Bonetta D, Finkelstein R, Provart NJ, Desveaux D, Rodriguez PL, McCourt P, Zhu JK, Schroeder JI, Volkman BF, Cutler SR

Author

Brian F. Volkman PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Abscisic Acid
Arabidopsis
Arabidopsis Proteins
Genes, Plant
Germination
Ligands
Membrane Transport Proteins
Mutation
Naphthalenes
Phosphoprotein Phosphatases
Protein Binding
Recombinant Fusion Proteins
Seeds
Signal Transduction
Sulfonamides
Two-Hybrid System Techniques
jenkins-FCD Prod-444 eb4ebd1a08581aba961d3befd3b851a3c3ec6b46