Identification of a nonneuronal isoform of synaptotagmin. Proc Natl Acad Sci U S A 1995 Jun 20;92(13):5895-9
Date
06/20/1995Pubmed ID
7597049Pubmed Central ID
PMC41608DOI
10.1073/pnas.92.13.5895Scopus ID
2-s2.0-0029036903 (requires institutional sign-in at Scopus site) 77 CitationsAbstract
Synaptotagmins, which have been found exclusively in neuroendocrine or exocrine tissues, have been implicated in the regulation of secretory vesicle fusion with the plasma membrane. The present paper describes a synaptotagmin isoform (synaptotagmin-5) which exhibits 49% amino acid identity to synaptotagmin-1 and -2. Synaptotagmin-5 mRNA is expressed in rat kidney, adipose tissue, lung, and heart, as well as at higher levels in brain and PC12 cells. Antiserum specific for the synaptotagmin-5 isoform recognizes a protein of about 50 kDa which is about 6-fold more abundant in brain synaptic vesicles than in whole brain membranes.
Author List
Hudson AW, Birnbaum MJAuthor
Amy W. Hudson PhD Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adipose TissueAmino Acid Sequence
Animals
Base Sequence
Brain
Calcium-Binding Proteins
Cell Line
Cloning, Molecular
Cricetinae
Gene Expression
Kidney
Lung
Membrane Glycoproteins
Molecular Sequence Data
Molecular Weight
Muscle, Skeletal
Myocardium
Nerve Tissue Proteins
Organ Specificity
PC12 Cells
Phylogeny
RNA, Messenger
Rats
Recombinant Proteins
Sequence Homology, Amino Acid
Synaptic Vesicles
Synaptotagmin I
Synaptotagmins
Transfection
