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odd-skipped related 2 is required for fin chondrogenesis in zebrafish. Dev Dyn 2013 Nov;242(11):1284-92

Date

08/06/2013

Pubmed ID

23913342

Pubmed Central ID

PMC4041527

DOI

10.1002/dvdy.24026

Scopus ID

2-s2.0-84886234377 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

BACKGROUND: odd-skipped related 2 (osr2) encodes a vertebrate ortholog of the Drosophila odd-skipped zinc-finger transcription factor. Osr2 in mouse is required for proper palate, eyelid, and bone development. Zebrafish knock-down experiments have also suggested a role for osr2, along with its paralog osr1, in early pectoral fin specification and pronephric development.

RESULTS: We show here that osr2 has a specific function later in development, independent of osr1, in the regulation of sox9a expression and promoting fin chondrogenesis. mRNA in situ hybridization demonstrated osr2 expression in the developing floorplate and later during organogenesis in the pronephros and gut epithelium. In the pectoral fin buds, osr2 was specifically expressed in fin mesenchyme. osr2 knock down in zebrafish embryos disrupted both three and five zinc finger alternatively spliced osr2 isoforms and eliminated wild-type osr2 mRNA. osr2 morphants exhibited normal pectoral fin bud specification but exhibited defective fin chondrogenesis, with loss of differentiated chondrocytes. Defects in chondrogenesis were paralleled by loss of sox9a as well as subsequent col2a1 expression, linking osr2 function to essential regulators of chondrogenesis.

CONCLUSIONS: The zebrafish odd-skipped related 2 gene regulates sox9a and col2a1 expression in chondrocyte development and is specifically required for zebrafish fin morphogenesis.

Author List

Lam PY, Kamei CN, Mangos S, Mudumana S, Liu Y, Drummond IA

Author

Pui Ying Lam PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animal Fins
Animals
Chondrogenesis
Gene Expression Regulation, Developmental
Transcription Factors
Zebrafish
Zebrafish Proteins