Corneal stroma PDGF blockade and myofibroblast development. Exp Eye Res 2009 May;88(5):960-5
Date
01/10/2009Pubmed ID
19133260Pubmed Central ID
PMC2674136DOI
10.1016/j.exer.2008.12.006Scopus ID
2-s2.0-64849109182 (requires institutional sign-in at Scopus site) 50 CitationsAbstract
Myofibroblast development and haze generation in the corneal stroma is mediated by cytokines, including transforming growth factor-beta (TGF-beta), and possibly other cytokines. This study examined the effects of stromal PDGF-beta blockade on the development of myofibroblasts in response to -9.0 diopter photorefractive keratectomy in the rabbit. Rabbits that had haze generating photorefractive keratectomy (PRK, for 9 diopters of myopia) in one eye were divided into three different groups: stromal application of plasmid pCMV.PDGFRB.23KDEL expressing a subunit of PDGF receptor b (domains 2-3, which bind PDGF-B), stromal application of empty plasmid pCMV, or stromal application of balanced salt solution (BSS). The plasmids (at a concentration 1000ng/microl) or BSS was applied to the exposed stroma immediately after surgery and every 24h for 4-5 days until the epithelium healed. The group treated with pCMV.PDGFRB.23KDEL showed lower alphaSMA+ myofibroblast density in the anterior stroma compared to either control group (P<or=0.001). Although there was also lower corneal haze at the slit lamp at one month after surgery, the difference in haze after PDGF-B blockade was not statistically significant compared to either control group. Stromal PDGF-B blockade during the early postoperative period following PRK decreases stromal alphaSMA+ myofibroblast generation. PDGF is an important modulator of myofibroblast development in the cornea.
Author List
Kaur H, Chaurasia SS, de Medeiros FW, Agrawal V, Salomao MQ, Singh N, Ambati BK, Wilson SEAuthor
Shyam S. Chaurasia PhD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ActinsAnimals
Corneal Opacity
Corneal Stroma
Female
Fibroblasts
Genetic Vectors
Photorefractive Keratectomy
Plasmids
Rabbits
Receptor, Platelet-Derived Growth Factor beta
Transfection