Expression of interleukin-1 receptor antagonist in human cornea. Exp Eye Res 2009 May;88(5):992-4
Date
12/18/2008Pubmed ID
19087877Pubmed Central ID
PMC2691719DOI
10.1016/j.exer.2008.11.019Scopus ID
2-s2.0-64849106124 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
The purpose of this study was to confirm the expression of interleukin-1 receptor antagonist (IL-1 Ra) in the human cornea. Four samples of human ex vivo corneal epithelium were obtained from patients undergoing photorefractive keratectomy. RT-PCR was performed using mRNA isolated from the corneal epithelium and oligo-dT primers. PCR was performed on the cDNA products using primers specific for human IL-1 Ra. The PCR products were subcloned and sequenced. Human cornea sections were prepared from eyes enucleated for choroidal melanoma. Immunocytochemistry was performed using goat anti-mouse polyclonal IL-1 Ra IgG and NL-577 conjugated donkey anti-goat IgG. IL-1 Ra mRNA was expressed in all ex vivo corneal epithelium samples as confirmed by sequencing of the PCR products. Immunofluorescence studies revealed strongest expression of IL-1 Ra in the superficial apical layer of corneal epithelium. Expression of IL-1 Ra may represent an endogenous mechanism of down-regulating the effects of epithelial- and tear-derived IL-1alpha and IL-1beta on the intact epithelium in the unwounded cornea and stromal cells after injury.
Author List
Heur M, Chaurasia SS, Wilson SEAuthor
Shyam S. Chaurasia PhD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Epithelium, CornealGene Expression
Humans
Interleukin 1 Receptor Antagonist Protein
Photorefractive Keratectomy