Dual regulation of cryptochrome 1 mRNA expression in chicken retina by light and circadian oscillators. Neuroreport 2002 Dec 03;13(17):2247-51
Date
12/19/2002Pubmed ID
12488805DOI
10.1097/00001756-200212030-00016Scopus ID
2-s2.0-0037016187 (requires institutional sign-in at Scopus site) 94 CitationsAbstract
The localization and regulation of chicken cryptochrome 1 (cCry1) mRNA expression in retina was investigated by laser capture microdissection and quantitative real-time RT-PCR. Laser capture microdissection (LCM) of retinal cell layers showed the highest level of cCry1 expression in the ganglion cell and photoreceptor layers. In both layers, expression was high during the daytime and low at night in subjects exposed to a 12:12 h light:dark cycle. Robust circadian oscillations of cCry1 mRNA levels were observed in constant (24 h day) light, but not in constant darkness, with the highest expression during daytime at zeitgeber time (ZT) 8. Unlike cCry1, circadian rhythms of the melatonin-synthesizing enzyme, arylalkylamine N-acetyltransferase, persisted in constant darkness, suggesting that rhythmic cCry1 expression is not essential for circadian clock function or output. On the second day of constant darkness, when cCry1 expression is arrhythmic, light exposure for 2 h significantly increased retinal cCry1 mRNA levels at ZT 4 and 8, times that cCry1 expression is induced in LD and LL. Similar light exposure ending at ZT 20 had no significant effect. Thus, expression of cCry1 mRNA is regulated dually by light and circadian clocks.
Author List
Haque R, Chaurasia SS, Wessel JH 3rd, Iuvone PMAuthor
Shyam S. Chaurasia PhD Professor in the Ophthalmology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adaptation, OcularAnimals
Arylamine N-Acetyltransferase
Biological Clocks
Chickens
Circadian Rhythm
Cryptochromes
Dark Adaptation
Drosophila Proteins
Eye Proteins
Flavoproteins
Gene Expression Regulation
Melatonin
Neurons
Photic Stimulation
Photoreceptor Cells
Photoreceptor Cells, Invertebrate
RNA, Messenger
Receptors, G-Protein-Coupled
Retina
Retinal Ganglion Cells
