Faculty Collaboration Database

Comparative study on effects of cytochalasins B and D on F-actin content in different cell lines and different culture conditions. Folia Histochem Cytobiol 1992;30(3):107-11

Date

01/01/1992

Pubmed ID

1337523

Scopus ID

2-s2.0-0027024609 (requires institutional sign-in at Scopus site)   23 Citations

Abstract

Effects of cytochalasins on actin polymerization state in living cells were measured using fluorimetry of TRITC-phalloidin bound to F-actin. Normal (3T3) and tumour (SV-3T3, B16 melanoma, and Ehrlich ascites) cells were treated with cytochalasin B and cytochalasin D (1 microgram/ml). Three effects of cytochalasins were demonstrated--depolymerization of F-actin, promotion of polymerization, and redistribution of actin without change in polymerization state. Occurrence of a given effect was dependent on cell type, cell density, cytochalasin concentration and type. This indicates that cells from different lines, and even the same cells in different culture conditions may differ significantly in their state of actin polymerization, which we suppose is the cause of their different reactions to cytochalasins. Accordingly, caution should be taken in generalizing the results concerning the effect of cytochalasis on the polymerization state of actin.

Author List

Wodnicka M, Pierzchalska M, Bereiter-Hahn J, Kajstura J

Author

Magdalena Chrzanowska PhD Associate Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

3T3 Cells
Actins
Animals
Carcinoma, Ehrlich Tumor
Cell Line, Transformed
Cytochalasin B
Cytochalasin D
Melanoma
Mice
Microscopy, Fluorescence
Polymers
Simian virus 40
Tumor Cells, Cultured