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Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy. Sci Rep 2021 Mar 04;11(1):5176

Date

03/06/2021

Pubmed ID

33664323

Pubmed Central ID

PMC7933323

DOI

10.1038/s41598-021-84543-9

Scopus ID

2-s2.0-85102042516 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

Studying the impact of antibiotic treatment on otitis media (OM), the leading cause of primary care office visits during childhood, is critical to develop appropriate treatment strategies. Tracking dynamic middle ear conditions during antibiotic treatment is not readily applicable in patients, due to the limited diagnostic techniques available to detect the smaller amount and variation of middle ear effusion (MEE) and middle ear bacterial biofilm, responsible for chronic and recurrent OM. To overcome these challenges, a handheld optical coherence tomography (OCT) system has been developed to monitor in vivo response of biofilms and MEEs in the OM-induced chinchilla model, the standard model for human OM. As a result, the formation of MEE as well as biofilm adherent to the tympanic membrane (TM) was longitudinally assessed as OM developed. Various types of MEEs and biofilms in the chinchilla model were identified, which showed comparable features as those in humans. Furthermore, the effect of antibiotics on the biofilm as well as the amount and type of MEEs was investigated with low-dose and high-dose treatment (ceftriaxone). The capability of OCT to non-invasively track and examine middle ear conditions is highly beneficial for therapeutic OM studies and will lead to improved management of OM in patients.

Author List

Won J, Hong W, Khampang P, Spillman DR Jr, Marshall S, Yan K, Porter RG, Novak MA, Kerschner JE, Boppart SA

Author

Ke Yan PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Anti-Bacterial Agents
Biofilms
Ceftriaxone
Chinchilla
Disease Models, Animal
Ear, Middle
Humans
Otitis Media
Otitis Media with Effusion
Tomography, Optical Coherence
Tympanic Membrane