Scanning ion conductance microscopy of live human glomerulus. J Cell Mol Med 2021 May;25(9):4216-4219
Date
03/22/2021Pubmed ID
33745233Pubmed Central ID
PMC8093965DOI
10.1111/jcmm.16475Scopus ID
2-s2.0-85102841283 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Podocyte damage is a hallmark of glomerular diseases, such as focal segmental glomerulosclerosis, typically associated with marked albuminuria and progression of renal pathology. Podocyte structural abnormalities and loss are also linked to minimal change disease and more common diabetic kidney disease. Here we applied the first-time scanning ion conductance microscopy (SICM) technique to assess the freshly isolated human glomerulus's topology. SICM provides a unique opportunity to evaluate glomerulus podocytes as well as other nephron structural segments with electron microscopy resolution but in live samples. Shown here is the application of the SICM method in the live human glomerulus, which provides proof of principle for future dynamic analysis of membrane morphology and various functional parameters in living cells.
Author List
Bohovyk R, Fedoriuk M, Isaeva E, Shevchuk A, Palygin O, Staruschenko AAuthor
Olena Isaeva PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
HumansKidney Glomerulus
Microscopy, Electron, Scanning
Podocytes
