Faculty Collaboration Database

Developmental delay of lingual lipase expression after guanethidine-induced sympathectomy. Proc Soc Exp Biol Med 1992 Feb;199(2):192-8

Date

02/01/1992

Pubmed ID

1741411

DOI

10.3181/00379727-199-43346

Scopus ID

2-s2.0-0026585448 (requires institutional sign-in at Scopus site)   6 Citations

Abstract

Rat lingual lipase increases during postnatal development. To evaluate the role of the sympathetic nervous system in the control of lingual lipase during development, suckling rats were chemically sympathectomized by chronic treatment with guanethidine. This treatment was found to be effective in suppressing the developmental increase of lingual lipase. The effect was age dependent and also related to the dose of guanethidine given (i.e., the higher the dose, the more effective the suppression is, up to 40 micrograms/g body wt). The effect of guanethidine on lingual lipase suppression was not a result of induced stress, since simultaneous treatment with RU-38486, a known glucocorticoid receptor antagonist, did not prevent the decrease in lingual lipase activity. Ephedrine, a known sympathomimetic agent, restored the lingual lipase to a near normal level in guanethidine-treated animals, confirming that guanethidine acts through the sympathetic nerves. Furthermore, histochemical studies showed that guanethidine-treatment resulted in the reduction or elimination of catecholaminergic fibers in the von Ebner's glands. The effect of guanethidine was found to be transient, in that the lingual lipase activity showed complete recovery upon withdrawal of the treatment for 1 week. Together, the results indicated that sympathetic nerves have an important regulatory role in lingual lipase in rat pups during development.

Author List

Lee PC, Purcell ES, Borysewicz R, Klein RM, Werlin SL

Author

Steven L. Werlin MD Emeritus Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Age Factors
Analysis of Variance
Animals
Animals, Suckling
Dose-Response Relationship, Drug
Ephedrine
Glucocorticoids
Guanethidine
Lipase
Mifepristone
Rats
Rats, Inbred Strains
Sympathectomy, Chemical
Sympathetic Nervous System
Tongue