Faculty Collaboration Database

Transcriptional analysis of the Pseudomonas aeruginosa exoenzyme S structural gene. J Bacteriol 1995 Mar;177(5):1169-78

Date

03/01/1995

Pubmed ID

7868588

Pubmed Central ID

PMC176720

DOI

10.1128/jb.177.5.1169-1178.1995

Scopus ID

2-s2.0-0028944578 (requires institutional sign-in at Scopus site)   87 Citations

Abstract

The transcriptional regulation of the Pseudomonas aeruginosa exoS gene was investigated. Expression of exoS in P. aeruginosa PA103 was dependent upon growth in a low-cation environment and the presence of a functional exsA gene. Promoter fusion analysis indicated that a 285-bp PstI-NsiI fragment, located 5' of the exoS coding region, contained a functional promoter for exoS. Expression of the reporter gene was inducible in a low-cation growth environment and required a functional copy of exsA. Divergent promoters, coordinately regulated with exoS transcription, were identified within the PstI-NsiI fragment. A fusion derivative of ExsA, MALA3A2, was shown to bind directly to the PstI-NsiI probe. DNase I protection analysis demonstrated that MALA3A2 bound to the intergenic region between the postulated -35 boxes of each promoter region. Northern (RNA) blot analysis with probes internal to and upstream of exoS demonstrated that separate, coordinately regulated mRNAs were expressed in P. aeruginosa. These data suggested that a locus, coregulated with exoS transcription, was located upstream of exoS. DNA sequence analysis of the exoS upstream region revealed three open reading frames, ORF 1, ORF 2, and ORF 3. ORF 1 demonstrated significant homology to the SycE/YerA protein of Yersinia sp. SycE/YerA is postulated to function as a chaperone for the YopE cytotoxin. The loci encoding YopE and ExoS show similarities in genetic organization, protein composition, and regulation.

Author List

Yahr TL, Hovey AK, Kulich SM, Frank DW

Author

Dara W. Frank PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

ADP Ribose Transferases
Amino Acid Sequence
Bacterial Outer Membrane Proteins
Bacterial Proteins
Bacterial Toxins
Base Sequence
DNA, Bacterial
DNA-Binding Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genetic Complementation Test
Molecular Sequence Data
Poly(ADP-ribose) Polymerases
Promoter Regions, Genetic
Protein Binding
Pseudomonas aeruginosa
Recombinant Fusion Proteins
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trans-Activators
Transcription, Genetic