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Oxidized low density lipoprotein increases U937 cell 5-lipoxygenase activity: induction of 5-lipoxygenase activating protein. Biochem Biophys Res Commun 1994 Jun 15;201(2):1014-20

Date

06/15/1994

Pubmed ID

8002971

DOI

10.1006/bbrc.1994.1803

Scopus ID

2-s2.0-0028308423   17 Citations

Abstract

This study examines the effects of oxidized low density lipoprotein (ox-LDL) on mononuclear cell arachidonic acid (AA) metabolism. U937 and HL60 cells, employed as models for mononuclear cells and promyelocytic cells, respectively, were exposed to ox-LDL (20 micrograms protein/ml) for 24 hours. HPLC analysis of 1-14C-AA metabolites indicates that ox-LDL increases U937 and HL60 cell production of 15-hydroxyeicosatetraenoic acid (15-HETE) and 5-hydroxyeicosatetraenoic acid (5-HETE). Northern analysis indicates that ox-LDL increases U937 cell FLAP transcript levels 10-times control levels but did not appear to alter 5-lipoxygenase (5-LO) mRNA levels. In contrast, ox-LDL increases HL60 cell transcript levels for FLAP and 5-LO 1.5 times and 10 times control levels, respectively. Thus, we propose that ox-LDL plays an important role in the up-regulation of the 5-LO pathway in mononuclear cells. Such activation may explain, in part, the mechanisms by which ox-LDL promotes atherogenesis.

Author List

Fair A, Pritchard KA Jr

Author

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

5-Lipoxygenase-Activating Proteins
Arachidonate 5-Lipoxygenase
Arachidonic Acid
Blotting, Northern
Carrier Proteins
Cell Line
Enzyme Activation
Gene Expression
Humans
Hydroxyeicosatetraenoic Acids
Kinetics
Leukemia
Lipoproteins, LDL
Lymphoma, Large B-Cell, Diffuse
Malondialdehyde
Membrane Proteins
Oxidation-Reduction
Precursor Cell Lymphoblastic Leukemia-Lymphoma
RNA, Neoplasm
Transcription, Genetic
Tumor Cells, Cultured
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a