Human Sertoli-spermatogenic cell cocultures prepared from biopsies of cryptorchid testes performed during orchidopexy. J Urol 1989 Apr;141(4):1003-9
Date
04/01/1989Pubmed ID
2564441DOI
10.1016/s0022-5347(17)41086-xScopus ID
2-s2.0-0024506351 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
A procedure is described for the preparation and maintenance of human Sertoli-spermatogenic cell cocultures using biopsies of normal and undescended testis. The evaluation of cell viability and differentiation potential of cultured spermatogenic cell was monitored by [3H]thymidine labeling combined with light microscopic autoradiography. Spermatogenic cells of the same progeny, connected by intercellular bridges, display synchronous DNA synthesis when labeled at the preleptotene stage of meiotic prophase. The pattern of [35S]methionine-labeled secretory proteins was determined by two-dimensional electrophoresis and autoradiography during testicular development and compared with these observed in human Sertoli-spermatogenic cell cocultures prepared from same specimens. Both testicular tissue and cocultured Sertoli and spermatogenic cells displayed comparable patterns of secretory proteins. A discrete group of acidic polypeptides of Sertoli cell origin enhanced their radiolabeling intensity during testicular development. Results of this paper indicate that human Sertoli-spermatogenic cell cocultures could be valuable for assessing the proliferation and differentiation potential of spermatogenic cells in children with cryptorchid testis.
Author List
Tres LL, Mesrobian HG, Abdullah M, Kierszenbaum ALAuthor
Hrair George Mesrobian MD Emeritus Professor in the Urology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
BiopsyCell Differentiation
Cell Survival
Cells, Cultured
Cryptorchidism
Cytological Techniques
Humans
In Vitro Techniques
Male
Sertoli Cells
Spermatogenesis
Testis
