Enhancement of merocyanine 540 uptake and photodynamic cell killing by salicylates. Cancer Res 1994 Jul 01;54(13):3474-8
Date
07/01/1994Pubmed ID
8012969Scopus ID
2-s2.0-0028234320 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
Salicylate and several structurally analogous compounds enhance merocyanine 540 (MC540)-photosensitized killing of leukemia cells (M. A. Anderson, B. Kalyanaraman, and J. B. Feix, Cancer Res., 53: 806-809, 1993). In this work, we show that salicylic acid enhances the binding of MC540 prior to illumination, as well as the light-stimulated uptake of MC540 by target L1210 murine and K562 human leukemia cells. Acetylsalicylic acid, 2,3- and 2,5-dihydroxybenzoic acids, and sodium benzoate also enhance MC540 uptake. The irradiation dose responses for loss of cell survival and enhanced MC540 uptake are well correlated, both being shifted to earlier time points in the presence of salicylate. Salicylic acid also enhanced photodynamic cell killing of A549 lung carcinoma and NIH:OVCAR-3 ovarian carcinoma cells, two cell types which are relatively resistant to MC540-mediated photosensitization. Cellular uptake of the anionic, potential-sensitive oxonol dye, bis-(1,3-dibutylbarbituric acid)-trimethine oxonol, is also increased by salicylate in a dose-dependent fashion. In contrast, cellular uptake of the cationic cyanine dye, 3,3'-dihexyloxacarbocyanine, is unaffected by salicylate. These studies suggest that increased uptake of MC540 is the basis of salicylate enhancement and that changes in plasma membrane potentials may play a mechanistic role in the potentiation of MC540 binding and cell killing.
Author List
Feix JB, Anderson MS, Kalyanaraman BAuthors
Jimmy B. Feix PhD Professor in the Biophysics department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBenzoates
Benzoic Acid
Cell Survival
Drug Screening Assays, Antitumor
Drug Synergism
Fluorescence
Humans
Leukemia
Leukemia L1210
Mice
Neoplasms
Photochemotherapy
Photosensitizing Agents
Pyrimidinones
Salicylates
Salicylic Acid