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Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid. Methods Mol Biol 2021;2275:315-327

Date

06/13/2021

Pubmed ID

34118047

Pubmed Central ID

PMC9245525

DOI

10.1007/978-1-0716-1262-0_20

Scopus ID

2-s2.0-85108205869 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

The development of boronic probes enabled reliable detection and quantitative analysis of hydrogen peroxide , other nucleophilic hydroperoxides, hypochlorite , and peroxynitrite . The major product, in which boronate moiety of the probe is replaced by the hydroxyl group, is, however, common for all those oxidants. Here, we describe how ortho-isomer of mitochondria-targeted phenylboronic acid can be used to detect and differentiate peroxynitrite-dependent and independent probe oxidation. This method highlights detection and quantification of both the major, phenolic product and the minor, peroxynitrite-specific cyclic and nitrated products of probe oxidation.

Author List

Zielonka J, Sikora A, Podsiadly R, Hardy M, Kalyanaraman B

Authors

Micael Joel Hardy PhD Visiting Assistant Professor in the Biophysics department at Medical College of Wisconsin
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
Jacek M. Zielonka PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Boronic Acids
Chromatography, High Pressure Liquid
Hydrogen Peroxide
Isotope Labeling
Mice
Mitochondria
Oxidation-Reduction
Peroxynitrous Acid
RAW 264.7 Cells
Tandem Mass Spectrometry