Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid. Methods Mol Biol 2021;2275:315-327
Date
06/13/2021Pubmed ID
34118047Pubmed Central ID
PMC9245525DOI
10.1007/978-1-0716-1262-0_20Scopus ID
2-s2.0-85108205869 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
The development of boronic probes enabled reliable detection and quantitative analysis of hydrogen peroxide , other nucleophilic hydroperoxides, hypochlorite , and peroxynitrite . The major product, in which boronate moiety of the probe is replaced by the hydroxyl group, is, however, common for all those oxidants. Here, we describe how ortho-isomer of mitochondria-targeted phenylboronic acid can be used to detect and differentiate peroxynitrite-dependent and independent probe oxidation. This method highlights detection and quantification of both the major, phenolic product and the minor, peroxynitrite-specific cyclic and nitrated products of probe oxidation.
Author List
Zielonka J, Sikora A, Podsiadly R, Hardy M, Kalyanaraman BAuthors
Micael Joel Hardy PhD Visiting Assistant Professor in the Biophysics department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
Jacek M. Zielonka PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBoronic Acids
Chromatography, High Pressure Liquid
Hydrogen Peroxide
Isotope Labeling
Mice
Mitochondria
Oxidation-Reduction
Peroxynitrous Acid
RAW 264.7 Cells
Tandem Mass Spectrometry