Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Expression of VACM-1 protein in cultured rat adrenal endothelial cells is linked to the cell cycle. Endothelium 2001;8(1):49-63



Pubmed ID




Scopus ID

2-s2.0-0035013794   16 Citations


The vasopressin-activated calcium-mobilizing (VACM-1) protein is a unique arginine vasopressin (AVP) receptor which shares sequence homology with the cullins, genes involved in the regulation of cell cycle transitions. Unlike either cullins or AVP receptors, however, VACM-1 is expressed exclusively in the vascular endothelial cells and in the renal collecting tubule cells. In order to test the hypothesis that the expression of VACM-1 might be correlated with the cell cycle, and to establish an endothelial cell model for the VACM-1 receptor, we examined VACM-1 expression in rat adrenal medulla endothelial cells (RAMEC). Northern and Western blot analyses of mRNA and protein from RAMEC identified presence of 6.4 kb mRNA and a Mr 81 kDa protein, respectively. Immunostaining of RAMEC with anti-VACM-1 antibodies and Western blot analyses indicated that in RAMEC, VACM-1 protein expression is dependent on the cell cycle. VACM-1 protein virtually disappears during the S phase and localizes to the cytosol during cell division and to the cell membrane at the completion of cytokinesis. Furthermore, pretreatment of RAMEC with anti-VACM-1 specific antibodies increased basal levels of Ca2+and attenuated the AVP-dependent increase in cytosolic Ca2+. In summary, these results indicate that VACM-1 protein expression in RAMEC membrane is linked to the cell cycle, and consequently, VACM-1 may be involved in the regulation of cell division.

Author List

Burnatowska-Hledin, Zeneberg A, Roulo A, Grobe J, Zhao P, Lelkes PI, Clare P, Barney C


Justin L. Grobe PhD Associate Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adrenal Medulla
Calcium Signaling
Cell Cycle
Cell Membrane
Cell Nucleus
Cells, Cultured
Cullin Proteins
Gene Expression Regulation
Membrane Proteins
Receptors, Vasopressin
Transcription, Genetic