Faculty Collaboration Database

Expression of VACM-1 protein in cultured rat adrenal endothelial cells is linked to the cell cycle. Endothelium 2001;8(1):49-63

Date

06/21/2001

Pubmed ID

11409851

DOI

10.3109/10623320109063157

Scopus ID

2-s2.0-0035013794 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

The vasopressin-activated calcium-mobilizing (VACM-1) protein is a unique arginine vasopressin (AVP) receptor which shares sequence homology with the cullins, genes involved in the regulation of cell cycle transitions. Unlike either cullins or AVP receptors, however, VACM-1 is expressed exclusively in the vascular endothelial cells and in the renal collecting tubule cells. In order to test the hypothesis that the expression of VACM-1 might be correlated with the cell cycle, and to establish an endothelial cell model for the VACM-1 receptor, we examined VACM-1 expression in rat adrenal medulla endothelial cells (RAMEC). Northern and Western blot analyses of mRNA and protein from RAMEC identified presence of 6.4 kb mRNA and a Mr 81 kDa protein, respectively. Immunostaining of RAMEC with anti-VACM-1 antibodies and Western blot analyses indicated that in RAMEC, VACM-1 protein expression is dependent on the cell cycle. VACM-1 protein virtually disappears during the S phase and localizes to the cytosol during cell division and to the cell membrane at the completion of cytokinesis. Furthermore, pretreatment of RAMEC with anti-VACM-1 specific antibodies increased basal levels of Ca2+and attenuated the AVP-dependent increase in cytosolic Ca2+. In summary, these results indicate that VACM-1 protein expression in RAMEC membrane is linked to the cell cycle, and consequently, VACM-1 may be involved in the regulation of cell division.

Author List

Burnatowska-Hledin, Zeneberg A, Roulo A, Grobe J, Zhao P, Lelkes PI, Clare P, Barney C

Author

Justin L. Grobe PhD Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adrenal Medulla
Animals
Aphidicolin
Calcium Signaling
Cell Cycle
Cell Membrane
Cell Nucleus
Cells, Cultured
Cullin Proteins
Endothelium
Gene Expression Regulation
Immunohistochemistry
Membrane Proteins
Rats
Receptors, Vasopressin
Transcription, Genetic