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Rotaviruses preferentially bind O-linked sialylglycoconjugates and sialomucins. Glycobiology 1993 Oct;3(5):437-45

Date

10/01/1993

Pubmed ID

8286856

DOI

10.1093/glycob/3.5.437

Scopus ID

2-s2.0-0027494242   39 Citations

Abstract

Rotaviruses are the most common cause of severe gastroenteritis in infants and children worldwide. Early events of virus binding and entry are the critical determinants of cellular permissiveness to rotavirus replication. The only known ligands for rotaviruses are sialic acids. We now report that simian rotaviruses bind preferentially to a subset of sialylated glycoconjugates, i.e. glycoproteins containing O-linked sialic acid moieties. Rotaviruses are able to distinguish between sialylated trisaccharide ligands presented as neoglycolipids. Higher avidity binding by rotaviruses is explained by multivalent binding to clustered sialic acid moieties. Our in vitro data are extended to explain the protective effect of mucins in the murine model of rotavirus disease and the specific binding by rotavirus to a high molecular weight sialomucin in the infant mouse intestine. Rotavirus binding to a sialomucin may be analogous to selectin-mediated mechanisms of cellular adhesion, and may be advantageous to the virus in the dynamic environment of the intestine.

Author List

Willoughby RE

Author

Rodney E. Willoughby MD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Binding Sites
Carbohydrate Sequence
Cattle
Child
Glycoconjugates
Guinea Pigs
Humans
In Vitro Techniques
Infant
Intestine, Small
Mice
Molecular Sequence Data
Molecular Weight
Mucins
Oligosaccharides
Rotavirus
Rotavirus Infections
Sialic Acids
Sialoglycoproteins
Sialomucins