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Thrombin modulates persistent sodium current in CA1 pyramidal neurons of young and adult rat hippocampus. Fiziol Zh (1994) 2015;61(4):5-10

Date

11/11/2015

Pubmed ID

26552299

DOI

10.15407/fz61.04.005

Scopus ID

2-s2.0-84952719146   1 Citation

Abstract

Serine protease thrombin, a key factor of blood coagulation, participates in many neuronal processes important for normal brain functioning and during pathological conditions involving abnormal neuronal synchronization, neurodegeneration and inflammation. Our previous study on CA3 pyramidal neurons showed that application ofthrombin through the activation of specific protease-activated receptor 1 (PAR1) produces a significant hyperpolarizing shift of the activation of the TTX-sensitive persistent voltage-gated Na+ current (I(Nap)) thereby affecting membrane potential and seizure threshold at the network level. It was shown that PAR1 is also expressed in CA1 area of hippocampus and can be implicated in neuronal damage in this area after status epilepticus. The aim of the present study was to evaluate the effect of thrombin on I(NaP) in CA1 pyramidal neurons from adult and young rats. Using whole cell patch-clamp technique we demonstrate that thrombin application results in the hyperpolarization shift of I(NaP) activation as well as increase in the I(NaP) amplitude in both age groups. We have found that I(NaP) in pyramidal neurons of hippocampal CA 1 region is more vulnerable to the thrombin action than I(NaP) in pyramidal neurons of hippocampal CA3 region. We have also found that the immature hippocampus is more sensitive to thrombin action which emphasizes the contribution of thrombin-dependent pathway to the regulation of neuronal activity in immature brain.

Author List

Lunko OO, Isaev DS, Krishtal OO, Isaeva EV

Author

Olena Isaeva PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Action Potentials
Aging
Animals
CA1 Region, Hippocampal
CA3 Region, Hippocampal
Gene Expression
Microtomy
Neurons
Organ Specificity
Patch-Clamp Techniques
Rats
Rats, Wistar
Receptor, PAR-1
Sodium
Tetrodotoxin
Thrombin
Tissue Culture Techniques
Voltage-Gated Sodium Channels