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Hyperbaric oxygen therapy and squamous cell carcinoma cell line growth. Head Neck 1993 May-Jun;15(3):236-40



Pubmed ID




Scopus ID

2-s2.0-0027471707   17 Citations


Hyperbaric oxygen (HBO) promotes tissue healing by increasing oxygenation. Therefore, HBO therapy is clinically useful for some patients who have undergone major cancer resection and/or radiotherapy to the head and neck. For individual patients, however, there might be undetected viable tumor present at the time of therapy. This study was performed to determine if increased tissue oxygen had a measurable effect on the growth of squamous carcinoma xenotransplants which had been derived from head and neck cancers. After the successful growth of two well-established human squamous cell carcinoma cell lines (183 and 1483), each tumor was transplanted into 20 mice. Every mouse received four transplants of 10(6) cells. Ten mice with 40 xenotransplants in each group were treated with HBO daily for 90 minutes at a pressure of 2 atm, whereas the other 10 formed the control group. The mice transplanted with cell line 1483 were treated for 21 days; mice transplanted with cell line 183 were treated for 28 days. The tumor weight, volume, and histology were evaluated. No significant difference was found between experimental groups. This study suggests that increased tissue oxygen neither significantly increases nor decreases the growth of squamous cell carcinoma.

Author List

Sklizovic D, Sanger JR, Kindwall EP, Fink JG, Grunert BK, Campbell BH


Bruce H. Campbell MD Professor in the Otolaryngology department at Medical College of Wisconsin
Brad K. Grunert PhD Professor in the Plastic Surgery department at Medical College of Wisconsin
James R. Sanger MD Professor in the Plastic Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Carcinoma, Squamous Cell
Head and Neck Neoplasms
Hyperbaric Oxygenation
Mice, Nude
Neoplasm Transplantation
Tumor Cells, Cultured