Faculty Collaboration Database

Use of the single-strand conformation polymorphism technique to detect loss of heterozygosity in neuroblastoma. Genes Chromosomes Cancer 1993 Jun;7(2):102-8

Date

06/01/1993

Pubmed ID

7687451

DOI

10.1002/gcc.2870070207

Scopus ID

2-s2.0-0027151846 (requires institutional sign-in at Scopus site)   34 Citations

Abstract

Human neuroblastomas are characterized by cytogenetic and molecular analysis as frequently containing deletions of distal 1p. Loss of heterozygosity (LOH) studies have localized a region of shared deletion to 1p35-36.1. Using the single-strand conformation polymorphism (SSCP) technique, we developed polymorphic assays for two genes, the amiloride-sensitive Na+/H+ antiporter (APNH) and tumor necrosis factor receptor 2 (TNFR2) genes, which map to this region. We used these SSCPs to detect LOH in a panel of neuroblastomas. Allelic loss was readily detected in 8 of 39 informative tumors. The SSCP-derived LOH results were consistent with LOH results generated from a set of distal 1p probes that identify restriction fragment length polymorphisms (RFLPs). The APNH locus could be excluded from the region of consistent deletion, but the TNFR2 locus could not be excluded. We conclude that the SSCP technique is a precise and efficient method for detecting LOH in human neoplasia.

Author List

White PS, Kaufman BA, Marshall HN, Brodeur GM

Author

Bruce A. Kaufman MD Adjunct Professor in the Neurosurgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Alleles
Amiloride
Base Sequence
Carrier Proteins
DNA, Single-Stranded
Gene Deletion
Heterozygote
Humans
Molecular Sequence Data
Neuroblastoma
Polymerase Chain Reaction
Polymorphism, Genetic
Receptors, Cell Surface
Receptors, Tumor Necrosis Factor
Sodium-Hydrogen Exchangers
Tumor Necrosis Factor-alpha