Faculty Collaboration Database

Age and gender effect on the inducibility of steroid metabolizing cytochrome P450 in rat liver. Biochem Mol Biol Int 1994 Nov;34(5):861-70

Date

11/01/1994

Pubmed ID

7703903

Scopus ID

2-s2.0-0028020553 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

Liver microsomal steroid hydroxylases and 5 alpha reductase activities were evaluated by quantitation of specific metabolites from 4-C14 progesterone after TLC separation. Each enzyme showed a different developmental profile depending on the gender of the rat. Dexamethasone induced both 6 beta and 16 alpha progesterone hydroxylase, being more potent for 6 beta (3 to 4 folds) than 16 alpha (1.2 to 1.6 folds). A comparison of the inducibility of 6 beta and 16 alpha hydroxylase by dexamethasone in rats from different age groups showed that for both enzymes, the degree of increase was higher in the younger than older groups. Thus there is a blunting in the responsiveness to dexamethasone induction of both 6 beta and 16 alpha hydroxylase with age particularly in female animals. This decrease in responsiveness in older females could potentially affect their capacity to metabolize endogenous and exogenous agents.

Author List

Lee PC, Struve MF, Werlin SL

Author

Steven L. Werlin MD Emeritus Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Cholestenone 5 alpha-Reductase
Cytochrome P-450 CYP2E1
Cytochrome P-450 Enzyme System
Dexamethasone
Enzyme Induction
Female
Isoenzymes
Male
Microsomes, Liver
Mixed Function Oxygenases
Oxidoreductases
Pregnancy
Rats
Rats, Sprague-Dawley
Sex Characteristics
Steroid 16-alpha-Hydroxylase