Medical College of Wisconsin
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Rapid identification from rectal swabs of the clinically most relevant carbapenemase genes from gram-negative bacteria using the BD MAX Check-Points CPO Assay. Diagn Microbiol Infect Dis 2022 Jan;102(1):115554

Date

10/23/2021

Pubmed ID

34678712

DOI

10.1016/j.diagmicrobio.2021.115554

Scopus ID

2-s2.0-85117421272 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

We conducted an international multicentre evaluation to assess the clinical performance characteristics of the new multiplex PCR-based BD MAX Check-Points CPO assay to detect the 5 major carbapenemase families: KPC, VIM/IMP (tested simultaneously), NDM and OXA-48 compared to a reference method consisting of 2 culture methods (to improve recovery of CPO isolates from the rectal swabs), followed by carbapenem susceptibility testing and sequencing of target carbapenemase genes. Tests were performed from rectal swab specimens in ESwab collection and transport devices. Positive percent agreement (PPA) for BD MAX Check-Points CPO for KPC and OXA-48 were 88.2% (95% CI:72.6-96.7) and 96.2% (95% CI:80.4-99.9), respectively. Negative percent agreement was ≥99% for each gene. Insufficient samples (≤10) were positive for VIM/IMP or NDM tests to calculate meaningful PPA values. The BD MAX Check-Points CPO assay represents an accurate tool for rapid recognition of patients with rectal colonization by the most commonly encountered CPOs.

Author List

García-Fernández S, Simner PJ, Thomson G, Faron M, Bosboom R, van Griethuijsen A, García-Castillo M, Harris R, Ledeboer NA, Cantón R, Thomson KS

Author

Nathan A. Ledeboer PhD Chief, Professor in the Pathology and Laboratory Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Anti-Bacterial Agents
Bacterial Proteins
Bacteriological Techniques
Carbapenems
Drug Resistance, Multiple, Bacterial
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Gram-Negative Bacteria
Humans
Multiplex Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Rectum
Sensitivity and Specificity
beta-Lactamases