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Adenovirus E1A and E1B-19K proteins protect human hepatoma cells from transforming growth factor beta1-induced apoptosis. Virus Res 2010 Jan;147(1):67-76

Date

10/27/2009

Pubmed ID

19854227

Pubmed Central ID

PMC2794482

DOI

10.1016/j.virusres.2009.10.008

Scopus ID

2-s2.0-71149104683   3 Citations

Abstract

Primary and some transformed hepatocytes undergo apoptosis in response to transforming growth factor beta1 (TGFbeta). We report that infection with species C human adenovirus conferred resistance to TGFbeta-induced apoptosis in human hepatocellular carcinoma cells (Huh-7). Protection against TGFbeta-mediated cell death in adenovirus-infected cells correlated with the maintenance of normal nuclear morphology, lack of pro-caspases 8 and 3 processing, maintenance of the mitochondrial membrane potential, and lack of cellular DNA degradation. The TGFbeta pro-apoptotic signaling pathway was blocked upstream of mitochondria in adenovirus-infected cells. Both the N-terminal sequences of the E1A proteins and the E1B-19K protein were necessary to protect infected cells against TGFbeta-induced apoptosis.

Author List

Tarakanova VL, Wold WS

Author

Vera Tarakanova PhD Associate Professor in the Microbiology and Immunology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adenovirus E1A Proteins
Adenovirus E1B Proteins
Adenoviruses, Human
Apoptosis
Cell Line
Hepatocytes
Humans
Transforming Growth Factor beta1
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a