Adenovirus E1A and E1B-19K proteins protect human hepatoma cells from transforming growth factor beta1-induced apoptosis. Virus Res 2010 Jan;147(1):67-76
Date
10/27/2009Pubmed ID
19854227Pubmed Central ID
PMC2794482DOI
10.1016/j.virusres.2009.10.008Scopus ID
2-s2.0-71149104683 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
Primary and some transformed hepatocytes undergo apoptosis in response to transforming growth factor beta1 (TGFbeta). We report that infection with species C human adenovirus conferred resistance to TGFbeta-induced apoptosis in human hepatocellular carcinoma cells (Huh-7). Protection against TGFbeta-mediated cell death in adenovirus-infected cells correlated with the maintenance of normal nuclear morphology, lack of pro-caspases 8 and 3 processing, maintenance of the mitochondrial membrane potential, and lack of cellular DNA degradation. The TGFbeta pro-apoptotic signaling pathway was blocked upstream of mitochondria in adenovirus-infected cells. Both the N-terminal sequences of the E1A proteins and the E1B-19K protein were necessary to protect infected cells against TGFbeta-induced apoptosis.
Author List
Tarakanova VL, Wold WSAuthor
Vera Tarakanova PhD Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adenovirus E1A ProteinsAdenovirus E1B Proteins
Adenoviruses, Human
Apoptosis
Cell Line
Hepatocytes
Humans
Transforming Growth Factor beta1
