Solving protein structure from sparse serial microcrystal diffraction data at a storage-ring synchrotron source. IUCrJ 2018 Sep 01;5(Pt 5):548-558
Date
09/19/2018Pubmed ID
30224958Pubmed Central ID
PMC6126656DOI
10.1107/S205225251800903XScopus ID
2-s2.0-85053159147 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
In recent years, the success of serial femtosecond crystallography and the paucity of beamtime at X-ray free-electron lasers have motivated the development of serial microcrystallography experiments at storage-ring synchrotron sources. However, especially at storage-ring sources, if a crystal is too small it will have suffered significant radiation damage before diffracting a sufficient number of X-rays into Bragg peaks for peak-indexing software to determine the crystal orientation. As a consequence, the data frames of small crystals often cannot be indexed and are discarded. Introduced here is a method based on the expand-maximize-compress (EMC) algorithm to solve protein structures, specifically from data frames for which indexing methods fail because too few X-rays are diffracted into Bragg peaks. The method is demonstrated on a real serial microcrystallography data set whose signals are too weak to be indexed by conventional methods. In spite of the daunting background scatter from the sample-delivery medium, it was still possible to solve the protein structure at 2.1 Å resolution. The ability of the EMC algorithm to analyze weak data frames will help to reduce sample consumption. It will also allow serial microcrystallography to be performed with crystals that are otherwise too small to be feasibly analyzed at storage-ring sources.
Author List
Lan TY, Wierman JL, Tate MW, Philipp HT, Martin-Garcia JM, Zhu L, Kissick D, Fromme P, Fischetti RF, Liu W, Elser V, Gruner SMAuthors
Wei Liu PhD Associate Professor in the Pharmacology and Toxicology department at Medical College of WisconsinLan Zhu PhD Assistant Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin
