Serial Femtosecond Crystallography of Membrane Proteins. Adv Exp Med Biol 2016;922:151-160
Date
08/25/2016Pubmed ID
27553241Pubmed Central ID
PMC5110035DOI
10.1007/978-3-319-35072-1_11Scopus ID
2-s2.0-84983781462 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals. Recent developments of serial femtosecond crystallography at X-ray free electron lasers combined with the use of membrane-mimetic gel-like matrix of lipidic cubic phase (LCP-SFX) for crystal growth and delivery hold significant promise to accelerate structural studies of membrane proteins. This chapter describes the development and current status of the LCP-SFX technology and elaborates its future role in structural biology of membrane proteins.
Author List
Zhu L, Weierstall U, Cherezov V, Liu WAuthors
Wei Liu PhD Associate Professor in the Pharmacology and Toxicology department at Medical College of WisconsinLan Zhu PhD Assistant Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Bacterial ProteinsCrystallization
Crystallography, X-Ray
Electrons
Humans
Lasers
Lipid Bilayers
Membrane Proteins
Receptors, G-Protein-Coupled
Synchrotrons
Temperature
Time Factors
