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Studying lipid organization in biological membranes using liposomes and EPR spin labeling. Methods Mol Biol 2010;606:247-69

Date

12/17/2009

Pubmed ID

20013402

Pubmed Central ID

PMC4640678

DOI

10.1007/978-1-60761-447-0_18

Abstract

Electron paramagnetic resonance (EPR) spin-labeling methods provide a unique opportunity to determine the lateral organization of lipid bilayer membranes by discrimination of coexisting membrane domains or coexisting membrane phases. In some cases, coexisting membrane domains can be characterized without the need for their physical separation by profiles of alkyl chain order, fluidity, hydrophobicity, and oxygen diffusion-concentration product in situ. This chapter briefly explains how EPR spin-labeling methods can be used to obtain the above-mentioned profiles across lipid bilayer membranes (liposomes). These procedures will be illustrated by EPR measurements performed on multilamellar liposomes made of lipid extracts from cortical and nuclear fractions of the fiber cell plasma membrane of a cow-eye lens. To better elucidate the major factors that determine membrane properties, results for eye lens lipid membranes and simple model membranes that resemble the basic lipid composition of biological membranes will be compared.

Author List

Subczynski WK, Raguz M, Widomska J

Author

Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cattle
Cell Membrane
Cell Membrane Permeability
Electron Spin Resonance Spectroscopy
Hydrophobic and Hydrophilic Interactions
Lens, Crystalline
Liposomes
Membrane Fluidity
Membrane Lipids
Membrane Microdomains
Oxygen