Faculty Collaboration Database

Genetically selected stem cells from human adipose tissue express cardiac markers. Biochem Biophys Res Commun 2007 Feb 16;353(3):665-71

Date

01/02/2007

Pubmed ID

17196165

DOI

10.1016/j.bbrc.2006.12.103

Scopus ID

2-s2.0-33846025383 (requires institutional sign-in at Scopus site)   59 Citations

Abstract

In the present study, the potential of human adipose-derived stem cells to differentiate into cells with characteristics of cardiomyocytes was investigated. Adipose tissue-derived stem cells (ADSCs) were transduced with two different lentiviral vectors simultaneously: (1) a lentiviral vector expressing eGFP controlled by the Nkx2.5 promoter and (2) a lentiviral vector expressing DsRed2 controlled by the myosin light chain-2v promoter (MLC-2v). Nkx2.5-eGFP and MLC-2v-DsRed2 dual positive cells were isolated by FACS. Immunostaining and RT-PCR analysis of the dual positive cells revealed that these cells are positive for Nkx2.5, cardiac troponin I, and L-type calcium channel alpha-1c subunit. Electrophysiology studies demonstrated the presence of functional voltage-dependent calcium and potassium channels. These observations confirm that cardiac progenitor cells can be isolated and enriched from human adipose-derived stem cells using lentiviral selection, and they might represent a new source for cell therapy for myocardial infarction and heart failure.

Author List

Bai X, Pinkernell K, Song YH, Nabzdyk C, Reiser J, Alt E

Author

Xiaowen Bai PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adipose Tissue
Calcium Channels, L-Type
Cell Differentiation
Flow Cytometry
Green Fluorescent Proteins
Humans
Lentivirus
Luminescent Proteins
Myocytes, Cardiac
Potassium Channels, Voltage-Gated
Stem Cells
Transduction, Genetic