The cardioprotective effect of mesenchymal stem cells is mediated by IGF-I and VEGF. Biochem Biophys Res Commun 2007 Nov 23;363(3):674-9
Date
10/02/2007Pubmed ID
17904522DOI
10.1016/j.bbrc.2007.09.058Scopus ID
2-s2.0-34848874465 (requires institutional sign-in at Scopus site) 290 CitationsAbstract
Emerging evidence suggests that adipose tissue-derived stem cells (ASCs) can be used for the treatment of ischemic heart diseases. However, the mechanisms underlying their therapeutic effects have not been clearly defined. In this study cytokines released by ASCs were detected by ELISA and pro-angiogenic effects were assessed by tube formation assay. To define the anti-apoptotic effect of ASCs, neonatal rat cardiomyocytes were subjected to hypoxia condition in a co-culture system. Our data show that ASCs secrete significant amounts of VEGF (810.65+/-56.92 pg/microg DNA) and IGF-I (328.33+/-22.7 pg/microg DNA). Cardiomyocytes apoptosis was significantly prevented by ASCs and 62.5% of the anti-apoptotic effect was mediated by IGF-I and 34.2% by VEGF. ASCs promoted endothelial cell tube formation by secreting VEGF. In conclusion we demonstrated that ASCs have a marked impact on anti-apoptosis and angiogenesis and helps to explain data of stem cells benefit without transdifferentiation.
Author List
Sadat S, Gehmert S, Song YH, Yen Y, Bai X, Gaiser S, Klein H, Alt EAuthor
Xiaowen Bai PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Newborn
Apoptosis
Cell Hypoxia
Cell Line
Cell Survival
Cells, Cultured
Coculture Techniques
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Humans
Insulin-Like Growth Factor I
Myocytes, Cardiac
Neovascularization, Physiologic
RNA, Messenger
RNA, Small Interfering
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Vascular Endothelial Growth Factor A
